FFPE RNA Isolation
The FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscles tissue. The sample is first processed in special buffer for deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While the FFPE RNA Kit is optimized to minimize the effect of the formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that requires full length RNA.