The EZgeneTM Tissue RNA Midi Kit provides an easy and fast method for isolating total RNA from cultured cells and animal tissues and within 60 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. This kit purifies up to 600 µg of total RNA from eukaryotic cells or animal tissues per column. Normally, up to 1E8 of eukaryotic cells and 250 mg of tissues can be processed per column. The purified RNA is ready for RT-PCR, Northern blotting, polyA+ RNA purification, nuclease protection, and in vitro translation.