The EZgeneTM Poly-Gel RNA Recovery Kit provides an easy and reliable method for recovering RNA probes from denaturing acrylamide gels. The probe is eluted by passive diffusion from the gel fragment with a unique buffer system and further purified using an ezBind RNA spin-column. The procedure can produce enough probes, ready for hybridization in 1-4 hours. The eluted RNA is ultra clean and ready for subsequent analysis and molecular manipulation.
Storage and Stability
All components of the Poly-Gel RNA Recovery Kit are stable for at least 24 months from the date of purchase when stored at 22-25oC. Once diluted, RNA Wash Buffer Concentrate must not be refrigerated.
Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.
- Add 16 mL (RC3612-00) or 96 mL (RC3612-01) or 320 mL (RC3612-02) 96%-100% ethanol to RNA Wash Buffer before use.
- Buffer *** may form crystals under cool ambient conditions. It is critical to warm up the buffers at 37oC to redissolve the salt.
-Add 20 µL of β-mercaptoethanol (β-mercaptoethanol) per 1 mL of Buffer RB before use. This mixture can be stored at room temperature up to 1 month.
All centrifugation steps must be carried out at room temperature.
Materials supplied by users
- 96%-100% ethanol
- Micro-centrifuge capable of 10,000 x g
- RNase-free microcentrifuge tubes and tips
- Disposable latex gloves