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Poly-Gel RNA Purification Kit
Qty / Pack : 20 Qty / Case : Download Document

  • Product Description
  • Details
The EZgeneTM Poly-Gel RNA Recovery Kit provides an easy and reliable method for recovering RNA probes from denaturing acrylamide gels. The probe is eluted by passive diffusion from the gel fragment with a unique buffer system and further purified using an ezBind RNA spin-column. The procedure can produce enough probes, ready for hybridization in 1-4 hours. The eluted RNA is ultra clean and ready for subsequent analysis and molecular manipulation.

Storage and Stability
All components of the Poly-Gel RNA Recovery Kit are stable for at least 24 months from the date of purchase when stored at 22-25oC. Once diluted, RNA Wash Buffer Concentrate must not be refrigerated.  

Before Starting
Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

- Add 16 mL (RC3612-00) or 96 mL (RC3612-01) or 320 mL (RC3612-02) 96%-100% ethanol to RNA Wash Buffer before use.
- Buffer *** may form crystals under cool ambient conditions. It is critical to warm up the buffers at 37oC to redissolve the salt.
-Add 20 µL of β-mercaptoethanol (β-mercaptoethanol) per 1 mL of Buffer RB before use. This mixture can be stored at room temperature up to 1 month.
 All centrifugation steps must be carried out at room temperature.

Materials supplied by users
- 96%-100% ethanol
- Micro-centrifuge capable of 10,000 x g
- RNase-free microcentrifuge tubes and tips
- Disposable latex gloves
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