The ViraTrapTM lentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Up to 3x1010 viral particles can be purified from cell culture media of 1 to 2 T75 flasks.
The viruses are first pelleted from viral supernatant and then further purified and concentrated through a purification column and a desalting/concentration unit.
Each column can be regenerated for purifying the same lentivirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
The Guaranteed shelf life is 12 months from the date of purchase. Store LV column at 4°C
Familiar with each step by reading this menu and prepare all materials for the procedure.
The lentiviral infected cell culture and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.
Materials required but not supplied
- Standard TC centrifuge
- Swing bucket rotor.
- 0.45 μm filter unit
- Rack holder for column