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HUVEC (human umbilical vein endothelial cell) Whole Cell Lysate
$100
Qty / Pack : 100ug Qty / Case : Download Document

  • Product Description
  • Details
Introduction
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Biomiga provides researchers with a variety of ready-to-use cell line lysates for proteomics research. Biomiga lysates are available for applications including electrophoresis, western blot, immunoprecipitation, enzymatic activity analysis, protein-protein interaction analysis, and tissue specific expression identification. Please check product documentation to see recommended and tested applications for each cell line lysate.
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Tested applications 
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WB
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Suggested applications 
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WB, ELISA, Immunoprecipitation
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Recommended dilutions
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HCC70 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
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Relevance 
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HCC70 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
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Purity 
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Whole Cell Lysate
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Form 
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It is supplied as whole cell lysate, in RIPA buffer with protease inhibitors and phosphotase inhibitors.
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Concentration 
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2.000 mg/ml
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Storage instructions 
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Shipped on dry ice. Upon delivery aliquot and store at  -80°C. Avoid repeated freeze / thaw cycles.
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Notes 
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Cell line: TOV-21G (Human ovarian adenocarcinoma)
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Growth media: M199/MCDB medium (1:1) with 15% fetal bovine serum.
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Figure 1. HUVEC cell morphology.
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Figure 2. GAPDH western blot using HUVEC lysate
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