Key to the kit is our proprietary DNA binding systems that allow the high efficient binding of DNA to our ezBindTM matrix while proteins and other impurities are removed by Wash Buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer.
Unlike other kits in the markets, our patented plasmid purification kit has no chaotropic salts in the buffer, the purified DNA is guanidine/anion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.
Copy numbers: The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 2 to 3 times. Reference the table below for the commonly used plasmids