GeneTranTM III is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) into eukaryotic cells providing the following advantages:
Highest transfection efficiency in many cell types and formats (e.g. 96-well).
Nucleic acid-GeneTranTM III complexes can be added directly to cells in culture medium, in the presence or absence of serum.
It is not necessary to remove complexes or change/add medium after transfection, but complexes may be removed after 4-6 hours.
Storage and Stability
The kit should be stored sterile at 4 °C (not -20 °C). It is stable for 12 months or longer.
Do not add antibiotics to media during transfection as this causes cell death.
Maintain the same seeding conditions between experiments.
Test serum-free media for compatibility with GeneTranTM III since some serum-free formulations (e.g. CD293, SFM II, VP-SFM) may inhibit cationic lipid-mediated transfection.