This kit enables researchers to prepare competent E. coli cells in a single step and to transform the cells without heat-shock. The competent cells obtained by this method can be kept at -70°C for long term storage. Transformation efficiencies depend on the strain of E. coli, as well as the nature and quality of the transforming DNA. Typical transformation efficiencies are 1E6~1E8 pfu/μg plasmid DNA.
- Entire procedure takes about 5 minutes.
- Suitable for long term storage at -80C.
- Cost effective: less than 10 cents /transformation