Key to the kit is our proprietary DNA binding systems that allow the high efficient binding of DNA to our ezBindTM matrix while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer.
Unlike other kits in the markets, our patented plasmid purification kit has no chaotropic salts in the buffer. The purified DNA is guanidine/anion exchange resin residues free.
Plasmid isolated with traditional protocol normally contains high level of endotoxins (Lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the endotoxins should be removed before the applications. The EZgeneTM endofree system uses a specially formulated buffer that extracts the endotoxin from the plasmid DNA. Two rounds of extraction will reduce the endotoxin level to 0.1 EU (Endotoxin) per µg of plasmid DNA. The endofree plasmid miniprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid DNA.
This kit is designed for fast and efficient purification of plasmid DNA from 15 to 50 mL of E. coli culture. The midi column has a DNA binding capacity of 250 µg.
The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection.
Plasmid Copy Numbers: The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 2 times. Please contact our customer service for further information and reference Table 1 for the commonly used plasmids