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ECL Western Blotting Substrate
$70
Qty / Pack : 25ml Qty / Case : Download Document

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ECL Western Blotting Substrate
Highersensitivity
Superiorsignal
Clearbackground
Easytouse

Procedure Summary
1.Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBS/T.

2.Prepare 1X ECL Western Blotting Substrate by mix Reagent A and Reagent B at a 1000:5 ratio (i.e., 5mL Reagent A + 25μL Reagent B for 6 × 8 cm2 membrane) and add it to the blot. 

3.Incubate Reagent with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic.

4.       Expose blot to X-ray film.
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