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Bacterial gDNA Kit
$20
Qty / Pack : 4 Qty / Case : Download Document

  • Product Description
  • Details
Introduction
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The EZgeneTM Bacterial gDNA kit provides a rapid and reliable method for isolating high-quality total cellular DNA from a wide variety of bacterial species. Bacterial cells are grown to log-phase and harvested. The bacterial cell wall is digested by lysozyme and Proteinase K. Following lysis, binding conditions are adjusted and the sample is applied to a DNA column.
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Key to this kit is our proprietary DNA binding system that allows the high efficient binding of DNA to our ezBindTM matrix while proteins and other contaminates are removed under certain optimal conditions. Nucleic acids are easily eluted with sterile water or Elution Buffer. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. Each DNA column can bind approximately 100 μg genomic DNA. Up to 1 x 109 bacterial cells can be processed per column. The system combines the reversible nucleic acid-binding properties of our ezBind matrix with the speed and versatility of spin column technology to yield up to approximately 15-30 μg of DNA with an OD260/280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA.
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Storage and Stability
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- The guaranteed shelf life for this kit is 12 months from the date of purchase.
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- Once reconstituted in water, Proteinase K and lysozyme must be stored at -20oC.
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- Store RNase A at 4oC.
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- Other components can be stored at 22oC-25oC.
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-Under cool ambient conditions, a precipitate may form in the Buffer BL/TL. In case of such an event, heat the bottle at 37oC to dissolve. Store Buffer BL/TL at room temperature.
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