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2x SBGR qPCR mix (Rox I)
Qty / Pack : 2.5 mL Qty / Case :

  • Product Description
  • Details
- It contains HS Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye.
- It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes.
- The chemically modified HS Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes.
- The unique PCR buffer components and the Hot start enzyme ensure PCR specificity and sensitivity.
- The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye.
- It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template.

Suitable Real Time PCR Detection Systems
- ABI Prism 7500/7500 Fast
- Strategene MX3000/MX3005P
- Corbett Rotor Gene 3000
- Systems need low ROX signals

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